Discover and read the best of Twitter Threads about #microglia
Most recents (17)
Thrilled to announce my 1st paper from @stevens1lab is now published @NatureNeuro. “Dissection of Artifactual and Confounding Glial Signatures by scRNA-seq of Mouse and Human Brain”. Why is it important? a 🧵👇 #singlecell #microglia #methodsmatter 1/n
nature.com/articles/s4159…
nature.com/articles/s4159…
While the title frames this work in terms of brain, a KEY takeaway from new data in this version is that this is broadly applicable across basically all scRNA-seq (and RNA-seq) studies (especially in immunology)... 2/n
...and the artifact we discuss is unfortunately highly prevalent in current literature. So stick around even if brain isn’t your thing 😉. We thoroughly characterize the issue and provide a robust flexible solution to eliminate it as well. 3/n
Does #gut #microbiota interact with #microglia in the #aging #brain? Are microglia metabolically affected (#mitochondria)? Does #diet take part? any #translational data?
Our paper made it last week in @NatureNeuro
so it's about time for a 🧵!
nature.com/articles/s4159…
Our paper made it last week in @NatureNeuro
so it's about time for a 🧵!
nature.com/articles/s4159…
1. Microglia from aged and young-adult #male & #female mice, under SPF or #germ_free conditions were profiled by bulk #RNAseq. We identified a gene-set, which depended on the housing condition regardless of #age & regulate central processes in microglia. "Microglial GF signature"
2. Utilizing #WGCNA, we found major differences between microglia of SPF and GF mice in the aged groups. But do we see any functional difference on a cellular level?
The receptors involved in SARS-CoV-2 infection are co-expressed in the cells of the NVU, especially in astrocytes and microglial cells. These receptors are functionally active as exposure of endothelial cells to the SARS CoV-2 S1 protein subunit ..
#HIV
#ACE2
#brain
#HIV
#ACE2
#brain
altered the expression pattern of tight junction proteins, such as claudin-5 and ZO-1. Additionally, HIV-1 infection upregulated ACE2 and TMPRSS2 expression in brain astrocytes and microglia cells.
#astrocytes
#microglia
#brain
#astrocytes
#microglia
#brain
SC2 in BBB mechanism.
1. Our work showing that GABA-receptive microglia selectively sculpt inhibitory synapses during development is out @CellCellPress: authors.elsevier.com/c/1dMWhL7PXe~wk
Wonderful collaboration w/ @stevens1lab @Datta_Lab Farhi lab @RichBonneauNYU @harvardmed @broadinstitute thread⬇︎
Wonderful collaboration w/ @stevens1lab @Datta_Lab Farhi lab @RichBonneauNYU @harvardmed @broadinstitute thread⬇︎
2. Work from many labs has shown that #microglia are key regulators of synapse #development but we know that not all #synapses are created equal. The best example? Excitatory vs inhibitory synapses. Are microglia a jack of all trades or do they focus on specific synapse types?
3. At the outset, we were unsure whether microglia even regulate the development of inhibitory synapses. The answer to this was "they do”. When we depleted microglia during cortical development, we observed increases in both excitatory and inhibitory synapses
Daily Bookmarks to GAVNet 05/07/2021 greeneracresvaluenetwork.wordpress.com/2021/05/07/dai…
America’s Forgotten History of Supervised Opioid Injection
undark.org/2021/04/29/for…
#OpioidAddiction #history #treatment
undark.org/2021/04/29/for…
#OpioidAddiction #history #treatment
Mathematics for gamblers
aeon.co/essays/a-mathe…
#mathematics #gambling #probability #philosophy #language
aeon.co/essays/a-mathe…
#mathematics #gambling #probability #philosophy #language
Our latest paper from @LabMetabolism is out! 🧠🔥
"Changes in mitochondrial morphology modulate LPS-induced loss of #calcium homeostasis in BV-2 microglial cells"
#Metabolism #NeuroTwitter #ImmunoTwitter
1/11
link.springer.com/article/10.100…
"Changes in mitochondrial morphology modulate LPS-induced loss of #calcium homeostasis in BV-2 microglial cells"
#Metabolism #NeuroTwitter #ImmunoTwitter
1/11
link.springer.com/article/10.100…
This was @osvaldo_rpe's first publication and a pretty cool debut indeed!
Osvaldo is a brilliant scientist that I had the honor to co-advise. No doubt I learned from him way way more than I taught.
A well-deserved first-authorship for an undergraduate student! 👏
2/11
Osvaldo is a brilliant scientist that I had the honor to co-advise. No doubt I learned from him way way more than I taught.
A well-deserved first-authorship for an undergraduate student! 👏
2/11
Go, go #mitochondria!
We already knew that microglial activation induces mitochondrial fragmentation and that mito morphology correlates with its Ca2+ uptake
But the influence of mito Ca2+ handling on #microglial activation was still unclear...
3/11
We already knew that microglial activation induces mitochondrial fragmentation and that mito morphology correlates with its Ca2+ uptake
But the influence of mito Ca2+ handling on #microglial activation was still unclear...
3/11
Excited to share first preprint from my postdoc work in Stevens lab: “Single Cell Sequencing Reveals Glial Specific Responses to Tissue Processing & Enzymatic Dissociation in Mice and Humans” a thread below #singlecell #microglia #methodsmatter 1/n
biorxiv.org/content/10.110…
biorxiv.org/content/10.110…
What started as small internal pilot in the lab, turned into full-fledged paper. I’ll detail some of the key findings in thread below. But first want to take a second and thank all of our fantastic colleagues and collaborators without whom this project wouldn’t be possible 2/n
Help from many fantastic members of Stevens lab @thammondglia , Lasse, Alec, @ConnorDufort , Sarah, @soyonhonglab and of course my mentor @stevenslab1. Continuing fantastic collaboration and advice from @macosko and his lab Tushar, Chuck, @Abdul_Squared , Naeem, and Velina. 3/n
We are thrilled to share our #preprint "Atlas of genetic effects in human #microglia transcriptome across brain regions, aging and #disease pathologies"!
Work led by @lopeskp @JackHumphrey_ #Gijsje and others from #deWitte and #Raj Lab (@towfiqueRaj)
t.ly/pZqQ 1/23
Work led by @lopeskp @JackHumphrey_ #Gijsje and others from #deWitte and #Raj Lab (@towfiqueRaj)
t.ly/pZqQ 1/23
Here, we describe the Microglia Genomic Atlas (#MiGA) project. A genetic and transcriptomic resource of 255 primary human microglia isolated from 4 brain regions of 100 donors with neurodegenerative and psychiatric disease and controls. 2/23
Cool paper detailing what body sites, cell types, symptoms #COVID-19 has been connected to thus far 👉 But one thing: everyone knows that most persistent #viral (and #bacterial) pathogens are capable of #infecting/driving an equally extensive # of #symptoms, right?
2/ For example, in this interview, Dharam Ablashi (who co-discovered the #virus HHV6) explains how HHV6 has been shown capable of contributing to #cancers, type 1 #diabetes, Hashimoto’s, #MS, HPA-axis dysregulation, ME/CFS, Alzheimer’s, neuroinflammation..microbeminded.com/2020/06/28/int…
3/ ..and that the HHV6 can survive in
#microglia, astrocytes, macrophage, neurons/nerves, #pancreatic islet cells, the lung, gut epithelial cells, the #liver etc etc etc!
#microglia, astrocytes, macrophage, neurons/nerves, #pancreatic islet cells, the lung, gut epithelial cells, the #liver etc etc etc!
I am really thrilled to release #AutoSpill onto @biorxivpreprint. It is a novel method for applying compensation to #flowcytometry data, which reduces the error by ~100,000-fold. It is thanks to AutoSpill that we can push machines to their max colours
biorxiv.org/content/10.110…
biorxiv.org/content/10.110…
#AutoSpill is a beautiful example of what #maths can add to #immunology, led by the talented Dr Carlos Roca.
It has really open up my eyes to the potential of #computationalbiology, and is the reason why we have a new #datascience position available:
babrahaminstitute.livevacancies.co.uk/#/job/details/…
It has really open up my eyes to the potential of #computationalbiology, and is the reason why we have a new #datascience position available:
babrahaminstitute.livevacancies.co.uk/#/job/details/…
So how does #AutoSpill work? If you just want to compensate your data, simply upload your single colour controls to autospill.vib.be and then copy the spillover matrix to your #flowcytometry program of choice
@CarlyEWhyte can walk you through the whole process in <2'
@CarlyEWhyte can walk you through the whole process in <2'
Study finds a “striking buildup of lipid droplets in microglia w/ aging in mouse/human #brains.” The cells, called ‘lipid-droplet-accumulating #microglia (LDAM), are defective in phagocytosis, produce high levels of ROS + secrete proinflammatory cytokines: nature.com/articles/s4159…
2/ Worth noting that persistent #viruses target #lipid signaling, synthesis + metabolism to remodel host cells like microglia into an environment optimal for their replication 👉 Indeed viral infection of LDAM cells cld explain ROS + proinflammatory state: ncbi.nlm.nih.gov/pmc/articles/P…
3/ Or since LPS induced lipid formation in the study, cld bacterial infection be involved? (ncbi.nlm.nih.gov/pmc/articles/P…) 👉 Especially w/ more teams reporting bacteria inside astrocytes/glia in the brain? (sciencemag.org/news/2018/11/d…) Any thoughts @wysscoray @CarolynBertozzi?
Team tests hypothesis that #FMT from aged donor mice into adult recipients cld impact #brain functions associated w/ #aging 👉 Find that FMT from aged donors led to impaired spatial learning and memory in adult recipients: biorxiv.org/content/10.110…
2/ FMT from aged mice also modulated expression of a variety of proteins implicated in maintenance of #synaptic plasticity + neurotransmission in the #hippocampus☝️and #microglia cells of the hippocampus fimbria acquired an aging-like phenotype
3/ The trends must be replicated/tested in humans! 👉 But, it makes my long-held contention that the #microbiome is involved in #aging processes less “crazy”😉 For more background check out this webinar I did on the topic w/ @mike_lustgarten + @subC0smos:
@AchucarroGlia organises an innovative concept of advanced training course for researchers focused or willing to work in the vibrant field of glial cells #GLIA #gliaSchool2020 🧠 🔍
Thread 👇
Thread 👇
This School fosters close interactions between speakers and attendees during three intense days.
With 🧬Practical 🧪 demonstrations of 🔬techniques and 📙 Brainstorming sessions. #gliaSchool2020
With 🧬Practical 🧪 demonstrations of 🔬techniques and 📙 Brainstorming sessions. #gliaSchool2020
#Euroglia 2019 in #Porto (PT) has just published the Meeting Program.
Very much looking forward to be there! #GliaMeeting #NetworkGlia #glia
glia2019.eu/_medien/_conte…
Very much looking forward to be there! #GliaMeeting #NetworkGlia #glia
glia2019.eu/_medien/_conte…
New in this edition:
To promote interactions between speakers and attendants, the program includes a session called “meet the speakers”. The sessions will take place during the lunch breaks from Thursday to Saturday; glia2019.eu/?s=meet-the-sp…
To promote interactions between speakers and attendants, the program includes a session called “meet the speakers”. The sessions will take place during the lunch breaks from Thursday to Saturday; glia2019.eu/?s=meet-the-sp…
What about the Posters in #Euroglia 2019 ??
How do #microglia successfully engulf + digest intracellular #pathogens/dead cells...while maintaning their dynamic behavior? 👉 Processing depends on shrinkage + packing of phagosomes into a unique cellular compartment called the gastrosome 👇👇👇
One reason this matters is b/c in most CNS #infection studies 👉 bacterial, viral + fungal pathogens tend to persist INSIDE neurons, #microglia + astrocytes (some examples below)☝️So gastrosome activity cld impact the ability of such cells to clear/recover from such infection
The topic is on my mind thanks to this paper 👉 The team identified several drug candidates that, in the lab, stimulated the innate #immune system to better eliminate pathogens from infected microglia☝️A potential treatment direction we must follow-up on!: ncbi.nlm.nih.gov/pmc/articles/P…
No need for skepticism of #microbiome + virome involvement in #ME/CFS 👉 But we must factor in the activity of organisms (bacteria, viruses, phages, fungi etc) that often persist + interact outside the gut☝️And many such organisms are not “good” but are pathogens/#pathobionts
In fact, Komaroff asked if anyone has a model for #ME/CFS that links together many findings recently presented at #MECFSatNIH19 👉 Well, that’s what my recent paper on ME/CFS does!...w/ #microbiome + virome activity central to how separate findings connect:frontiersin.org/articles/10.33…
That is b/c 👉 Humans are now understood to be #holobionts: our activity is controlled by the human genome, our microbial/#viral genomes and their respective proteins + metabolites working in tandem (together!): nph.onlinelibrary.wiley.com/doi/full/10.11…
Paper proposes that changes to the maternal gut #microbiome during #pregnancy alter levels of microbial #metabolites (including neurotransmitters + neuropeptides)👉 If these metabolites cross the placental barrier, they may impact fetal #brain development:ncbi.nlm.nih.gov/pmc/articles/P…
It follows that gut #microbiome dysbiosis in the mother may change how #microbial metabolites regulate + prime infant #microglia and other neuroimmune cells 👉 This dysregulation may contribute to the development of neurodevelopmental disorders including #autism:
Quote from paper 👉 “We address an emerging #hypothesis behind neurodevelopmental disorders, which implicates #microbiome dysbiosis in the atypical programming of neuroimmune cells, namely microglia”: ncbi.nlm.nih.gov/pmc/articles/P…
