Discover and read the best of Twitter Threads about #AutoSpill
Most recents (2)
For anyone doing #flowcytometry, would you like to have a new protocol that reduces your #antibody costs by ~10-fold and also gives you higher quality data, with better signal-to-noise ratio?
Yes? Then read work by @olivertburton and @CarlyEWhyte. 1/6
…rentprotocols.onlinelibrary.wiley.com/doi/epdf/10.10…
Yes? Then read work by @olivertburton and @CarlyEWhyte. 1/6
…rentprotocols.onlinelibrary.wiley.com/doi/epdf/10.10…
Bottom-line-up-front: stain overnight. Antibody staining is sensitive to both dilution and time, so a titration that gives crummy staining in 30' can actually give beautiful staining overnight. The best part is, that lower dilution gives less background, for better separation 2/6
You can see this quite clearly with even tricky staining, like #Foxp3 and #cytokines. The overnight stain gives a stronger positive signal without increasing the background at all. Plus your cells are ready in the morning, when the cytometer is free, rather than 8pm at night! 3/6
I am really thrilled to release #AutoSpill onto @biorxivpreprint. It is a novel method for applying compensation to #flowcytometry data, which reduces the error by ~100,000-fold. It is thanks to AutoSpill that we can push machines to their max colours
biorxiv.org/content/10.110…
biorxiv.org/content/10.110…
#AutoSpill is a beautiful example of what #maths can add to #immunology, led by the talented Dr Carlos Roca.
It has really open up my eyes to the potential of #computationalbiology, and is the reason why we have a new #datascience position available:
babrahaminstitute.livevacancies.co.uk/#/job/details/…
It has really open up my eyes to the potential of #computationalbiology, and is the reason why we have a new #datascience position available:
babrahaminstitute.livevacancies.co.uk/#/job/details/…
So how does #AutoSpill work? If you just want to compensate your data, simply upload your single colour controls to autospill.vib.be and then copy the spillover matrix to your #flowcytometry program of choice
@CarlyEWhyte can walk you through the whole process in <2'
@CarlyEWhyte can walk you through the whole process in <2'
