Discover and read the best of Twitter Threads about #Foxp3
Most recents (4)
For anyone doing #flowcytometry, would you like to have a new protocol that reduces your #antibody costs by ~10-fold and also gives you higher quality data, with better signal-to-noise ratio?
Yes? Then read work by @olivertburton and @CarlyEWhyte. 1/6
…rentprotocols.onlinelibrary.wiley.com/doi/epdf/10.10…
Yes? Then read work by @olivertburton and @CarlyEWhyte. 1/6
…rentprotocols.onlinelibrary.wiley.com/doi/epdf/10.10…
Bottom-line-up-front: stain overnight. Antibody staining is sensitive to both dilution and time, so a titration that gives crummy staining in 30' can actually give beautiful staining overnight. The best part is, that lower dilution gives less background, for better separation 2/6
You can see this quite clearly with even tricky staining, like #Foxp3 and #cytokines. The overnight stain gives a stronger positive signal without increasing the background at all. Plus your cells are ready in the morning, when the cytometer is free, rather than 8pm at night! 3/6
Recently, we published the concluding part III of the #SPIRAL model, which we were working on since 2016.
Pathological T Helper Polarization Requires Pre-existing Cross-Reactive Memory T Cells
I'm going to explain the significance of this model.
preprints.org/manuscript/202…
Pathological T Helper Polarization Requires Pre-existing Cross-Reactive Memory T Cells
I'm going to explain the significance of this model.
preprints.org/manuscript/202…
In part I published in 2018, we introduced the SPIRAL model. It proposes that cross-reactivity is the basic unit that drives the evolution of adaptive immune system and it is thymus-derived regulatory T cells, Tregs, that rely on cross-reactive epitopes to control other T cells
We also proposed that thymus-derived #Foxp3 Tregs rely on #CrossReactive epitope derived from the friendly gut microbiota to survive and function in the periphery. So one of the main roles of gut microflora is to supply #Tregs with epitopes.
onlinelibrary.wiley.com/doi/10.1111/sj…
onlinelibrary.wiley.com/doi/10.1111/sj…
I am really thrilled to release #AutoSpill onto @biorxivpreprint. It is a novel method for applying compensation to #flowcytometry data, which reduces the error by ~100,000-fold. It is thanks to AutoSpill that we can push machines to their max colours
biorxiv.org/content/10.110…
biorxiv.org/content/10.110…
#AutoSpill is a beautiful example of what #maths can add to #immunology, led by the talented Dr Carlos Roca.
It has really open up my eyes to the potential of #computationalbiology, and is the reason why we have a new #datascience position available:
babrahaminstitute.livevacancies.co.uk/#/job/details/…
It has really open up my eyes to the potential of #computationalbiology, and is the reason why we have a new #datascience position available:
babrahaminstitute.livevacancies.co.uk/#/job/details/…
So how does #AutoSpill work? If you just want to compensate your data, simply upload your single colour controls to autospill.vib.be and then copy the spillover matrix to your #flowcytometry program of choice
@CarlyEWhyte can walk you through the whole process in <2'
@CarlyEWhyte can walk you through the whole process in <2'
a removal of a subset of #Tregs called T follicular regulatory cells (Tfr) from the immune system in #Foxp3-cre Bcl6-fl/fl mice #paradoxically reduces, rather than increases, #peanut-specific #IgE responses.
#immunology
bit.ly/2RAMe8L
#immunology
bit.ly/2RAMe8L
And if you think maybe their knockout mice are some kind of weirdos, not really. Their model also shows that total IgE is increasing as expected. So, the system the authors are using is within acceptable norms.
bit.ly/2RAMe8L
bit.ly/2RAMe8L
The authors then went on to show that IL-10 derived from Tfr cells are important for promoting peanut allergy-producing IgE production. (IL-10 is lesser understood cytokine but it is generally accepted as an immunosuppressant.) #immunology
