Discover and read the best of Twitter Threads about #superresolution
Most recents (6)
#quantum is here! Why not collect #fluorescence #photon-by-photon in your laser-scanning #microscope? We developed the #BrightEyes_TTM, an #opensource data acquisition system able to do it!
nature.com/articles/s4146…
@IITalk @ERC_Research @RNA_iit @MSCActions #BrightEyes_ERC
(1/5)
nature.com/articles/s4146…
@IITalk @ERC_Research @RNA_iit @MSCActions #BrightEyes_ERC
(1/5)
Our time-tagging #DAQ module can collect single-photon signals from 25 independent channels and reference signals from 3 channels. We showed how this module combined with a #SPAD array allows us to perform #FLISM and #FLFS. (2/5)
#imagescanningmicroscopy #fluorescence #lifetime
#imagescanningmicroscopy #fluorescence #lifetime
We characterized the #BrightEyes_TTM. We can reach a single-shot precision of 30 ps, 4 ns dead-time, and an "infinite" range in all channels, with a total flux of up to 125 MHz. (3/5)
#TCSPC #antibunching #quantum #microscopy #FPGA
#coding #LIDAR
#TCSPC #antibunching #quantum #microscopy #FPGA
#coding #LIDAR
Vector-based SARS-CoV2 #vaccines have been associated with vaccine-induced thrombotic thrombocytopenia #VITT/#TTS. It is known that similar to HIT, anti-PF4 antibodies are formed which activate platelets therefore eventually leading to serious thrombosis: nature.com/articles/s4158…
This serious and sometimes fatal condition has been described for both vector-based SARS-CoV2 vaccines ChAdOx1 nCoV-19 (AstraZeneca) and in less frequency for Ad26.COV2.S (Johnson&Johnson). However, it is still unclear why there are large differences in the incidence of #VITT.
To tackle this question, we dropped everything else and compared both vaccines in our local, highly interdisciplinary multi-expertise group from @uni_greifswald, @UMGreifswald and @Loeffler_News: #GreifswalderForscher
tinyurl.com/yw6sk5uz
1/9 Could graphene coverslips improve your single-molecule fluorescence and superresolution experiments? In our article in @AdvancedAms we combine #DNAorigami with #graphene to perform #singlemolecule #biophysics, #biosensing & #superresolution microscopy.
1/9 Could graphene coverslips improve your single-molecule fluorescence and superresolution experiments? In our article in @AdvancedAms we combine #DNAorigami with #graphene to perform #singlemolecule #biophysics, #biosensing & #superresolution microscopy.
2/9 Graphene is almost transparent and quenches fluorescence in a range of ~40 nm. Fluorescence intensity or fluorescence lifetime of single molecules directly tell you the distance of a molecule to the surface. Differences in the z-direction as small as 2.9 nm are resolved.
3/9 Dynamics with graphene energy transfer (GET). We use GET to visualize motion on top of a graphene surface and determine the timescale of fluctuations via autocorrelation analysis.
Excited to introduce #3DRCAN for denoising, super resolution and expansion microscopy. Super proud of this colab with Hari Shroff, Jiji Chen et al.
@NIBIBgov @arpcomplex
Preprint: biorxiv.org/content/10.110…
Code: github.com/AiviaCommunity…
#microscopy #aimicroscopy #aivia
1/17
@NIBIBgov @arpcomplex
Preprint: biorxiv.org/content/10.110…
Code: github.com/AiviaCommunity…
#microscopy #aimicroscopy #aivia
1/17
"Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes"
J Chen, H Sasaki, H Lai, Y Su, J Liu, Y Wu, A Zhovmer, C Combs, I Rey-Suarez, H Chang, C Huang, X Li, M Guo, S Nizambad, A Upadhyaya, J Lee, L Lucas, H Shroff.
2/17
J Chen, H Sasaki, H Lai, Y Su, J Liu, Y Wu, A Zhovmer, C Combs, I Rey-Suarez, H Chang, C Huang, X Li, M Guo, S Nizambad, A Upadhyaya, J Lee, L Lucas, H Shroff.
2/17
We are particularly interested in characterizing the limits of #deeplearning based approaches to image restoration. You will find several experiments comparing several best-in-class approaches: #3DRCAN, #CARE, #SRResNet and #ESRGAN.
#microscopy #aimicroscopy #iSIM
3/17
#microscopy #aimicroscopy #iSIM
3/17
(1/6) Excited to share that our OligoFISSEQ paper is up (nature.com/articles/s4159…)! We set out to develop single cell in situ #3Dgenome mapping methods. A fun collaborative effort led by @huy_sauce @ShyamtanuC and David Castillo, from @TingWu_Lab and @mamartirenom groups.
(2/6) An idea that began with @SonCNguyen and Evan Daugharthy. The rest of the team including: @GuyNir5 @AntoniosLioutas @ElliotHershberg @Nuno_MCMartins @PaulReginato Mohammed Hannan @oligopain and @geochurch . Shout out to everyone @naturemethods for the great experience!
(3/6) To target and visualize genomic regions at genome-wide scale, we barcoded Oligopaints, increasing target capacity exponentially. We then visualize and map the targets by #InSituSequencing the barcodes using hybridization, synthesis, and ligation-based approaches.
#Pumpy paper is out @NatureComms - "Automating Multimodal Microscopy with NanoJ-Fluidics"! We developed an open-source inexpensive #LEGO-based fluidics system that can be coupled to a microscope to achieve high-performance imaging nature.com/articles/s4146…
We created video abstract with @LaineBioImaging and @christlet that quickly explains paper. #Pumpy enables live-to-fixed imaging, fixation-on-event, super-resolution sequential-labelling (unlim. number protein species), it adds a new dimension to what’s possible at the microscope
@LaineBioImaging @christlet @palmada86 @P_Matos_Pereira did an excellent job making #Pumpy happen. An excellent collaboration with the Charras, @BaumBuzz and @christlet labs. Kudos to @christlet, despite the distance we interacted almost on a daily basis getting the paper together
