Discover and read the best of Twitter Threads about #fluorescence

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Multiplexed barcoding of cells:

Fluorescence Polarization Spectroscopy
pubmed.ncbi.nlm.nih.gov/34163769/
en.wikipedia.org/wiki/Fluoresce…

https://www.warsash.com.au/products/optoelectronics/RECEIVER-MODULES.php https://www.warsash.com.au/suppliers/dataray.php
Image
I fucking knew it. That's just wonderful. 😑
Read 64 tweets
#quantum is here! Why not collect #fluorescence #photon-by-photon in your laser-scanning #microscope? We developed the #BrightEyes_TTM, an #opensource data acquisition system able to do it!
nature.com/articles/s4146…
@IITalk @ERC_Research @RNA_iit @MSCActions #BrightEyes_ERC
(1/5) Image
Our time-tagging #DAQ module can collect single-photon signals from 25 independent channels and reference signals from 3 channels. We showed how this module combined with a #SPAD array allows us to perform #FLISM and #FLFS. (2/5)
#imagescanningmicroscopy #fluorescence #lifetime Image
We characterized the #BrightEyes_TTM. We can reach a single-shot precision of 30 ps, 4 ns dead-time, and an "infinite" range in all channels, with a total flux of up to 125 MHz. (3/5)
#TCSPC #antibunching #quantum #microscopy #FPGA
#coding #LIDAR Image
Read 5 tweets
So you want to do ammonia-free Birch reductions?

“Large Scale Synthesis of Dysprosium and Neodymium Diiodides” (plus Thulium 💚).

pubs.acs.org/doi/abs/10.102…

A thread #ChemTwitter #RealTimeChem (but from the past). (Table - pubs.acs.org/doi/10.1021/ja…)
Divalent lanthanide chemistry is often limited to Sm(II), Eu(II) and Yb(II). Eu(II) is actually stable in degassed water, the other two are oxidized. SmI2 is a powerful reductant used lots in organic chemistry.

Eu(II) has some pretty #Fluorescence!!
Now, Sm, Eu and Yb behave nicely and you can just take the metal carefully with I2 in THF to make [LnI2(THF)5], which can be partially desolvated. In that picture above the inner blue is [EuI2(THF)5], and the outer pale blue is EuI2(THF)2.

You can also use I-C2H4-I, or I2CH2.
Read 13 tweets

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