Discover and read the best of Twitter Threads about #mesoSPIM
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The CompassLSM: an #opensource #mesoSPIM-inspired #lightsheet #Microscope excitation path that allows 3.5 um axial resolution over a 10 mm FOV using axially scanned lightsheet microscopy osapublishing.org/boe/fulltext.c… 
My main comment about this paper is about publication strategy and not science: I probably should not have called it #opensource: "CAD file available upon request" - it should be easier than that! Don't make it so hard for biologists to copy your work!
If you want your technology adopted, make adoption really, really easy - find a vendor for parts kits, provide the complete parts list, provide software, provide documentation - don't let users do a lot of the legwork. Lowering the barrier to entry is key.
We are excited to share the mesoSPIM, a versatile open-source light-sheet microscope for imaging in cleared tissue. #mesoSPIM @openSPIM #lightsheet
Check out our webpage: mesospim.org
Check out our webpage: mesospim.org
The mesoSPIM is compatible with all clearing techniques and was tested with
active and passive CLARITY, CUBIC, BABB, iDISCO and MASH.
active and passive CLARITY, CUBIC, BABB, iDISCO and MASH.
The key mesoSPIM feature is axially scanned light-sheet microscopy (ASLM) introduced by @RetoPaul and @kD3AN which allows uniform axial resolution across large FOVs. To translate the light-sheet, we are using tunable lenses from
@optotunecompany
@optotunecompany
