Discover and read the best of Twitter Threads about #lightsheet
Most recents (4)
How do you image the large and the small at the same time? We developed new 🔬 technology to image centimeter-scale specimens - including whole mouse brains 🧠- with diffraction-limited resolution and without sectioning. #mesoscale #imaging
biorxiv.org/content/10.110…
🧵 (1/n)
biorxiv.org/content/10.110…
🧵 (1/n)
ExA-SPIM is a technology based on innovations in (1) tissue processing and (2) large-scale microscopy 🔬. Our team developed new methods for expansion for microscopy (ExM) of large tissue volumes. #teamscience #expansionmicroscopy
protocols.io/view/whole-mou…
🧵 (2/n)
protocols.io/view/whole-mou…
🧵 (2/n)
By combining ExM with a new SPIM system based on technologies from electronics metrology, ExA-SPIM pushes past the volumetric imaging barrier 🚧 that constrains previous imaging approaches. (inspired by recent plot from @Daetwyler_St, @RetoPaul) #lightsheet #microscopy
🧵 (3/n)
🧵 (3/n)
The CompassLSM: an #opensource #mesoSPIM-inspired #lightsheet #Microscope excitation path that allows 3.5 um axial resolution over a 10 mm FOV using axially scanned lightsheet microscopy osapublishing.org/boe/fulltext.c… 
My main comment about this paper is about publication strategy and not science: I probably should not have called it #opensource: "CAD file available upon request" - it should be easier than that! Don't make it so hard for biologists to copy your work!
If you want your technology adopted, make adoption really, really easy - find a vendor for parts kits, provide the complete parts list, provide software, provide documentation - don't let users do a lot of the legwork. Lowering the barrier to entry is key.
We just updated our preprint, reporting a novel single-objective #lightsheet design that is capable of imaging large samples, with a large imaging volume, uncompromized image resolution and speed, and multi-view imaging. (1/9)
doi.org/10.1101/2020.0…
doi.org/10.1101/2020.0…
Thanks to @DrLachie, now we have a visual abstract which explains how the microscope works. First half of the movie below. (2/9)
Second half of the visual abstract by @DrLachie (3/9)
We are excited to share the mesoSPIM, a versatile open-source light-sheet microscope for imaging in cleared tissue. #mesoSPIM @openSPIM #lightsheet
Check out our webpage: mesospim.org
Check out our webpage: mesospim.org
The mesoSPIM is compatible with all clearing techniques and was tested with
active and passive CLARITY, CUBIC, BABB, iDISCO and MASH.
active and passive CLARITY, CUBIC, BABB, iDISCO and MASH.
The key mesoSPIM feature is axially scanned light-sheet microscopy (ASLM) introduced by @RetoPaul and @kD3AN which allows uniform axial resolution across large FOVs. To translate the light-sheet, we are using tunable lenses from
@optotunecompany
@optotunecompany
