Most recents (3)

Adam Glaser

@adam_k_glaser

How do you image the large and the small at the same time? We developed new 🔬 technology to image centimeter-scale specimens - including whole mouse brains 🧠 - with diffraction-limited resolution and without sectioning. #mesoscale #imaging

biorxiv.org/content/10.110…

🧵 (1/n)

ExA-SPIM is a technology based on innovations in (1) tissue processing and (2) large-scale microscopy 🔬. Our team developed new methods for expansion for microscopy (ExM) of large tissue volumes. #teamscience #expansionmicroscopy

protocols.io/view/whole-mou…

🧵 (2/n)

By combining ExM with a new SPIM system based on technologies from electronics metrology, ExA-SPIM pushes past the volumetric imaging barrier 🚧 that constrains previous imaging approaches. (inspired by recent plot from @Daetwyler_St, @RetoPaul) #lightsheet #microscopy

🧵 (3/n)

Read 12 tweets

Ons M'Saad

@OnsMSaad1

Introducing #panExM-t: a protocol where 🧠 tissue is expanded ✨24x✨, pan-stained with NHS ester, and immunolabeled.
Now you can see everything in the brain, even synaptic densities 👇. High potential for #connectomics!
bioRxiv: biorxiv.org/content/10.110…

Here's a data set showing synapses, mitochondria, and spine apparatuses

We found synapse morphology can be classified according to their NHS ester pan-staining patterns - similar to PTA staining in EM (Gray)

Read 7 tweets

Daniel Witvliet

@dwitvliet

How do brains rewire throughout life?

Our paper out in @Nature today outlines several principles of brain circuit development that we found by mapping the complete wiring of eight brains.

nature.com/articles/s4158…

#neuroscience #connectomics #brain #neurons #celegans #science