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Exciting work from @KathyYWei1, @AmeyaHarmalkar & @proteinrosh on predicting scFv developability. TLDR: transformers and CNNs can potentially help prioritise mutations sites for enhancing stability (1/5) #antibodies #machinelearning #proteineng
Context: a single-chain Fv (scFv) is an antibody construct whose heavy and light chains are linked. It's not the conventional "Y" shape molecule, and is useful for engineering / phage display, etc. See @AlissaHummer's post blopig.com/blog/2021/07/a… (2/5)
Thermostability (measured by TS50, the temperature when scFv loses binding) is weakly predicted by 0-shot and fine-tuning via transformers (ESM-1v + ESM-1b). CNNs using sequence and structural (energy) convolutions perform better (?) [hard to tell, sorry!🙈] (3/5)
