Discover and read the best of Twitter Threads about #assembloids
Most recents (4)
From our recent @Nature— a classification [part 4]
‘#Organoids can be used to form #assembloids…’
‘The combination of organoids with other organoids or with different specialized cell types in self-organizing 3D cultures results in emergent features of the system..’
‘#Organoids can be used to form #assembloids…’
‘The combination of organoids with other organoids or with different specialized cell types in self-organizing 3D cultures results in emergent features of the system..’
‘Assembloids are obtained by: incorporating different organoids into multi-region assembloids, such as when combining dorsal with ventral forebrain, cerebral cortex with striatum, or cerebral cortex with thalamus; integrating neural or non-neural cells…’
‘Assembloids can be generated from organoids derived from different individuals, to create inter-individual assembloids, or different species, to create inter-species assembloids’
Self-organizing systems have been one of the most exciting recent advances in stem cell research
However, many names & classifications are used making it challenging to convey the science
We now got together as a field to provide a nomenclature framework
Out in @Nature today👇1/9
However, many names & classifications are used making it challenging to convey the science
We now got together as a field to provide a nomenclature framework
Out in @Nature today👇1/9
We focused on neural #organoids #assembloids & #transplantation
Our consensus is summarized in one simple figure (above) & a short text box 👇
Our consensus is summarized in one simple figure (above) & a short text box 👇
Hundreds of genes have been associated with autism & neurodev disorders
But how do these genes impact specific stages in human development?
In a new preprint, @XianglingM took a bold approach & developed CRISPR screens in #assembloids to map 425 genes on interneuron dev stages 👇
But how do these genes impact specific stages in human development?
In a new preprint, @XianglingM took a bold approach & developed CRISPR screens in #assembloids to map 425 genes on interneuron dev stages 👇
To achieve this, @XianglingM & our team generated and screened over 1000 forebrain #assembloids built from parts derived from human pluripotent stem cells
This approach revealed that 46 out of a 425 autism spectrum disorder & other neurodevelopmental disease genes (~11%) expressed in the ventral forebrain interfere with either human GABAErgic cortical interneuron generation or interneuron migration
Thrilled to share new work from my lab @Stanford in @NatureBiotech today on generating functional cortico-striatal #assembloids, striatal #organoids & using them to model disease. Work led by the amazing @myym_yuki with help from @Minyin_Li @fikribirey @omerevah @TheteMayuri.
Check out the human medium spiny neurons:
and the cortical neurons (green) projecting in cortico-striatal #assembloids
